Super-resolution optical microscopy, commonly referred to as optical nanoscopy, has enabled imaging of biological samples with a resolution that was only achievable previously using electron microscopy. Optical nanoscopy is a rapidly growing field, with several different techniques and implementations that overcome the diffraction limit of light. However, the common nanoscope continues to be a rather ...

Photonic chip-based total internal reflection fluorescence microscopy (c-TIRFM) is an emerging technology enabling a large TIRF excitation area decoupled from the detection objective. Additionally, due to the inherent multimodal nature of wide waveguides, it is a convenient platform for introducing temporal fluctuations in the illumination pattern. The fluorescence fluctuation-based nanoscopy technique ...

In this project it was found that Fourier ptychographic microscopy can be improved far beyond its conventional limits via waveguide-based optical systems. Extensive in silico studies showed that images obtained on highrefractive index material waveguide chips in conjunction with hyperspectral illumination light and finely designed waveguide geometries can be combined via a modified phase-retrieval ...

Correlative light and electron microscopy (CLEM) unifies the versatility of light microscopy (LM) with the high resolution of electron microscopy (EM), allowing one to zoom into the complex organization of cells. Here, we introduce photonic chip assisted CLEM, enabling multi-modal total internal reflection fluorescence (TIRF) microscopy over large field of view and high precision localization of the ...

We report a methane sensor based on an integrated Mach-Zehnder interferometer, which is cladded by a styrene-acrylonitrile film incorporating cryptophane-A. Cryptophane-A is a supramolecular compound able to selectively trap methane, and its presence in the cladding leads to a 17-fold sensitivity enhancement. Our approach, based on 3 cm-long low-loss Si3N4 rib waveguides, results in a detection limit ...

Structured illumination microscopy (SIM) enables live-cell super-resolution imaging of subcellular structures at high speeds. At present, linear SIM uses free-space optics to illuminate the sample with the desired light patterns; however, such arrangements are prone to misalignment and add cost and complexity to the microscope. Here, we present an alternative photonic chip-based two-dimensional SIM ...

Labelfree nanoscopy encompasses optical imaging with resolution in the 100 nm range using visible wavelengths. Here, we present a labelfree nanoscopy method that combines coherent imaging techniques with waveguide microscopy to realize a <i>super-condenser</i> featuring maximally inclined coherent darkfield illumination with artificially stretched wave vectors due to large refractive indices of the ...

Large fields of view (FOVs) in total internal reflection fluorescence microscopy (TIRFM) via waveguides have been shown to be highly beneficial for single molecule localisation microscopy on fixed cells [1,2] and have also been demonstrated for short‐term live‐imaging of robust cell types [3‐5], but not yet for delicate primary neurons nor over extended periods of time. Here, we present a waveguide‐based ...